Mutation of the Trp92 that is known to lie within the active site of the photoprotein obelin from Obelia longissima, results in a shift of the bioluminescence color from blue (λ m a x =485 nm) to violet. The corrected spectrum shows a new band with λ m a x =410 nm now contributing equally to the one at longer wavelength. The crystal structure of this W92F obelin determined at 1.72 Å resolution shows that there is no significant change in the dimensions of the active site between WT obelin (recombinant Ca 2 + -regulated photoprotein from Obelia longissima) and the mutant. It is proposed that the bioluminescence spectral shift results from removal of a hydrogen bond from the indole of W92 nearby a hydroxyl belonging to the 6-phenyl substituent of the substrate coelenterazine. Propagation of this change through a conjugated bond system in the excited state of the product coelenteramide affects the coupling of the N1-position and the hydrogen-bonded Y138.