The biorefinery concept study for Jatropha curcas Linn (JCL) have been conducted by Bumimas Ekapersada team since 2010. This paper reports study of substrate hydrolysis retention time optimization and augmentation in two stage digestion from raw husk capsules (DH-JCL). It was conducted at the research farm of PT Bumimas Ekapersada, Bekasi, West Java, Indonesia. The augmentation treatment was comparation of 5% local commercial decomposer (EM-4) and arachea methanogens (GP-7) than digester slurry of DH-JCL. It concludes biogas production based on DH-JCL can be enhanced by retention time of hidrolysis substrate on 4 and/or 7 days, and using similar slurry starter.