Turkey carcasses were sampled in one commercial poultry processing plant of Isfahan (Iran) to evaluate the contamination of carcasses to Campylobacter spp. along the processing plant by PCR method. A total of 348 samples were collected during six plants visit from May to December 2007. Campylobacter spp. were isolated from 62.1% (216 out of 348) of the samples. Out of 216 Campylobacter isolates, 175 (81.0%) were identified as Campylobacter jejuni and 41 (19.0%) as Campylobacter coli. The occurrences of Campylobacter spp. contamination after defeathering, evisceration and chilling were 75.9%, 77.6% and 32.8% respectively. The results indicate that the fecal recontamination of the avian skin occurred during defeathering and evisceration. However chilling process decreases Campylobacter spp. contamination of the turkey skin significantly.