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A continuous cell line (SISK) from kidney of sea bass, Lates calcarifer, has been established and characterized. The cell line was maintained in Leibovitz' L-15 supplemented with 15% fetal bovine serum. This cell line has been subcultured more than 100 times over a period of 2 years. The SISK cell line consists of predominantly of epithelial-like cells. These cells showed strong positive for epithelial...
The susceptibility of three species of marine shrimp (Penaeus indicus, Penaeus japonicus and Penaeus monodon) to Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV) was tested by oral route and intramuscular injection. The results revealed that these marine shrimp were not susceptible to these viruses which failed to produce mortality in shrimp. RT-PCR analysis revealed the presence...
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