A theoretical study is presented of the dependence on the hydroxymethyl group conformation of vicinal carbon-proton coupling constant 3 J C , H in a series of 16 hexopyranoses. Calculated 3 J C , H values for both anomers of d-glucopyranose (1), d-mannopyranose (2), d-allopyranose (3),d -altropyranose (4), d-galactopyranose (5), d-talopyranose (6),d -gulopyranose (7), and d-idopyranose (8) are based on the FTP formulation in the semi-empirical approximation of INDO. The dependence of the coupling constants on the dihedral angle ω C between the coupling carbon atom C-4 and protons H-6 is represented by a trigonometric function of the form 3 J C , H = 5.8cos 2 ω C - 1.6cosω C + 0.28sin2ω C - 0.02sinω C + 0.52. It was found that the configuration at the anomeric and C-4 carbon atoms does not show any significant influence on 3 J C , H values. Agreement of calculated and experimental values available for mono- and oligo-saccharides is satisfactory. Based on these results, it is concluded that proposed equation for 3 J C , H values can be used as a tool for estimation of the conformational properties of the hydroxymethyl group in monosaccharides and of (1 → 6)- linked oligosaccharides in solution.