This study was to explore the effects of surfactant on biochemical and hydrothermal conversion of hemicellulose into biofuels and value-added co-products. It was found that extraction of loblolly pine with 0.4% H 2 SO 4 (w/w) at 150°C for 2h can dissolve most of hemicellulose. The addition of surfactant (0.4% w/w) showed significant improvement on hemicellulose extraction, hemicellulose yield increased from 8.14±0.16% to 10.15±0.02% (w/w) based on raw biomass. Pre-extracted hemicellulose was fermented to ethanol by Saccharomyces cerevisiae. In the presence of 0.4% surfactant, the glucose consumption rate in the hydrolysate fermentation increased from 0.28gg −1 h −1 to 0.46gg −1 h −1 , and mannose consumption rate increased from 0.30gg −1 h −1 to 0.35gg −1 h −1 . The final ethanol concentration increased from 7.45±0.18g/L to 10.68±0.26g/L. It was also observed that the hemicellulose extract could be effectively converted to levulinic acid at low pH (∼2) by a hydrothermal process. Under the condition of 200°C for 45min, 38.61±1.06% of hexoses from the hydrolysate extracted by 0.4% of sulfuric acid converted to HMF, and 64.48±1.20% of pentoses to furfural. The addition of surfactant (0.4% w/w) significantly increased the selectivity of furan by 41% for HMF and 30% for furfural.