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Recombinant human gastric lipase (rHGL) (EC 3.1.1.3) was produced on a large scale (5–13 mg/liter) from recombinant baculovirus-infected insect cells using a bioreactor apparatus. Here an improved procedure is described for purifying rHGL involving the use of cation exchange chromatography followed by immunoaffinity column methods, which gives a total yield of 62% and a purification factor of 464,...
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