Forensic techniques are becoming more and more powerful and affordable. This allows labs to utilise precise strategies, permitting multiple analytical approaches on the same evidence, thus obtaining precious information to solve criminal cases. This paper refers to a murder in which we received a plastic bottle and four latex gloves. These items were collected near a stolen car used to perpetrate the murder, and then burnt in order to destroy evidence linked to the murderer. We collected samples of saliva from the neck of the bottle and one glove underwent three different analyses, which were:•Sampling and genetic analyses of sweat traces taken from the internal surface of the glove, corresponding to the lower palm area;•Detection of palm-prints from the internal surface of the glove, corresponding to the upper palm area;•Collection of gun shot residues (GSR) from the edge of the glove.Two full genetic profiles were obtained from the biological traces collected, one from the glove and the other one from the bottle. The analyses were instrumental in permitting the identification of the shooter who had played an important role in the murder.