Chiral recognition capability of (+)-(R)-18-crown-6-tetracarboxylic acid (18C6H 4 ) to various amino compounds containing 16 amino acids, five alkyl amines, seven aminoalcohols and other amino compounds in nuclear magnetic resonance ( 1 H-NMR) analysis was investigated. In general, amino compounds having an aromatic ring were well chiral recognized with 18C6H 4 compared with those having no aromatic ring. Effects of 18C6H 4 concentration and the kind of deuterated solvents (D 2 O, CD 3 OD and CD 3 CN) for measurement on the chiral recognition was investigated in detail. Concentration of 5 equivalent 18C6H 4 to the amino compounds was found to be sufficient for the chiral recognition. On the other hand, an effective deuterated solvent (D 2 O, CD 3 OD or CD 3 CN) for measurement was different in each compound. Distinguishment of 1.0% of the minor enantiomer (d-form) in l-alanine-β-naphthylamide was found to be possible by 1 H-NMR employing 18C6H 4 as a chiral shift reagent.