Tool-chip and tool-work adhesion often limit machinability, but quantitative methods to characterise adhesion are absent. Hence, a methodology based on interrupted turning was used and layer formation was quantified using a number of techniques. This included surface topography studies using 3D white light interferometry, element mapping and profiling using SEM-EDS and Laser ablation time of flight mass spectrometry (LA -TOFMS) along with temperature mapping using IR-CCD. Results from turning tests with stainless steel 316L indicate clearly that the above techniques compliment each other and provide valuable new insight on contact friction, adhesion and layer formation.