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A sensitive enzyme-linked immunosorbent assay (ELISA) was developed for the detection of the herbicide trifluralin. Polyclonal antibodies were developed in rabbits against a haptenic analogue N-(2,6-dinitro-4-trifluoromethylphenyl)-N-n-propyl-6-aminohexanoic acid. Using antibodies against this hapten, a hapten-heterologous competitive ELISA in the antigen-immobilized format allowed detection of the...
A highly sensitive immunosensor using optical waveguide lightmode spectroscopy (OWLS) was developed for the detection of the herbicide trifluralin. OWLS as an in situ and label free method of detection, based on the measurement of the diffraction of a linearly polarized laser beam (He-Ne laser, 632.8nm) on a diffraction grating in a thin waveguide layer (SiO 2 -TiO 2 ), offered means...
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