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Based on a high affinity monoclonal antibody, an enzyme-linked immunosorbent assay (ELISA) was developed for the quantification of carbaryl (1-napththyl N-methylcarbamate). For standards, the detection limit of the ELISA was 0.065 ng ml -1 , with a I 50 value of 0.706 ng ml -1 . The ELISA was applied to the determination of carbaryl in apple and grape juices...
Monoclonal antibodies (MAbs) for the carbamate pesticide carbofuran (2,3-dihydro-2,2-dimethyl-7-benzofuranyl methylcarbamate) were obtained by immunizing mice with the hapten 3-[[(2,3-dihydro-2,2-dimethyl-7-benzofuranyloxy) carbonyl]amino] propanoic acid (BFNP) conjugated to bovine serum albumin (BSA). Based on one of these MAbs in combination with an heterologous hapten, an indirect enzyme-linked...
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