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During the reversible reaction between peroxidase (HRP) and peroxides, several peroxidase intermediate species, showing different molecular absorption spectra, are formed which can be used for their determination. On this basis, a reversible reagentless optical biosensor based on HRP for hydrogen peroxide and peracetic acid determinations has been developed. The biosensor (which can be used for at...
A novel mechanism to detect hydrogen peroxide (H 2 O 2 ) using a poly(N-butyl benzimidazole) (PBBI)-modified gold (PBBI/Au) electrode is proposed. Synthetic PBBI was oxidized using a mixture of acetic acid (AcOH) and H 2 O 2 to form PBBI N-oxide (PBBINO). The structure of PBBINO was verified by Fourier transform infrared spectroscopy (FT-IR) and the degree of oxidation...
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