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Simple fluorescence flow-injection immunoassays have been developed for theophylline (a hapten) and albumin (a macromolecule) using a thiophilic affinity matrix (T-gel). This gel is far superior to the previously used bacterial immunoglobulin binding proteins (e.g., proteins A and G) in terms of cost, elution conditions, non-specific binding and specificity for immunoglobulins regardless of type...
A fluorimetric method for the determination of low levels of theophylline based on the inhibition of the enzyme activity of alkaline phosphatase is proposed. The approach used is a flow-injection configuration where bovine alkaline phosphatase is immobilized on controlled pore glass. The enzymatic reaction is based on the hydrolysis of 4-methylumbelliferone phosphate with fluorimetric monitoring...
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