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A number of fluorescein derivatives were synthesized and their fluorescent properties were studied. Diphosphates of fluorescein derivatives were tested for the alkaline phosphatase-linked fluorescence assay of membrane-bound DNA to give high resolution detection of DNA. DNA was found to be detectable down to 20 fg in an assay using N-(2-biphenylcarbonyl)- and N-(4-biphenylcarbonyl)-5-aminofluorescein...
An in situ photochemical fluorescence probe method for the determination of DNA with sodium 9,10-anthraquinone-2-sulfonate (AQS) as a photochemical fluorescence probe was developed. It was based on the conversion of AQS into an intensively fluorescent product by irradiating with UV radiation. The photochemical reaction is retarded by DNA. The determination can be carried out by measuring the fluorescence...
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