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Fumonisins are a group of naturally occurring mycotoxins produced by several species of the fungus Fusarium (mainly moniliforme) and occur worldwide on corn intended for human and animal consumption. Contamination of food and feed with fumonisins has been implicated in and associated with a number of diseases in both livestock as well as human beings. In this study, a Flow-Injection Liposome ImmunoAnalysis...
Methods for the synthesis of fumonisin B1 (FmB1) immunoconjugates and for the preparation of FmB1-tagged liposomes were developed and studied. Keyhole limpet hemocyanin-fumonisin B1 (KLH-FmB1) was synthesized using both the classical glutaraldehyde (GA) protocol and a modified 2-step GA protocol. The immunoconjugate was then used to raise polyclonal antibodies in Rambouilet sheep. The covalent coupling...
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