Mitochondria dynamics results from fission and fusion events that may be unbalanced in favor of mitochondrial fragmentation upon cell stress. During oxidative stress, microtubules are hyperacetylated in a mitochondria-dependent manner. In this study, we show that under stress conditions, most of the mitochondria form foci with microtubule domains that carry Drp1. We also demonstrate that stress-induced hyperacetylation of microtubules is required for the effective induction of Drp1 phosphorylation at 616Ser, in a kinesin-1- and c-Jun N-terminal kinase-dependent manner. Furthermore, hyperacetylation of microtubules contributes to the recruitment of total Drp1 to mitochondria to enhance fission. These results highlight a new way of interaction between microtubules and mitochondria dynamics.