The lasing condition and frequency stability of the single longitudinal mode oscillation of a diode laser pumped 2 μm Tm,Ho:YLF microchip laser at room temperature are reported. It is shown that the microchip laser with an output mirror of 99.0% reflectivity had better single longitudinal mode performance than that with an output mirror of 99.5% reflectivity. The frequency tuning rate when varying the crystal temperature was estimated to be 1.9 GHz/ o C. Frequency stability of the microchip laser is examined by the self-beating heterodyne detection method for several delay times between 0.48 and 4.8 μs. It is indicated that the spectral fluctuation is in proportion to the delay time and the increasing rate is 2.3 kHz/μs.