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Conditions for zymographic detection of a 41-kDa spore cortex hydrolysis-specific autolysin, A6, from Bacillus subtilis 168 were optimised. A6 was present during sporulation from stages II-IV and remained active in the dormant spore. Its expression was controlled by the mother cell-specific early-sporulation sigma factor σ E . The characteristic muramic acid δ-lactam of spore cortical peptidoglycan...
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