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Bacillus subtilis vegetative cells undergo autolysis when exposed to cold shock treatment. A mutant (CA1) resistant to cold shock was isolated, and its DNA was used for the transformation of B. subtilis 168AR. The transformant (TR1) and CA1 had almost completely lost major vegetative autolysins (CwlB and CwlG) and motility, and showed a filamentous cell morphology during the exponential phase. Expression...
Conditions for zymographic detection of a 41-kDa spore cortex hydrolysis-specific autolysin, A6, from Bacillus subtilis 168 were optimised. A6 was present during sporulation from stages II-IV and remained active in the dormant spore. Its expression was controlled by the mother cell-specific early-sporulation sigma factor σ E . The characteristic muramic acid δ-lactam of spore cortical peptidoglycan...
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