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A choline uptake system accumulating free choline in an energy-dependent process is described in Mycoplasma fermentans. The uptake system has a K m of 2.2×10 −5 M and a V max of 0.15 nmol 10 min −1 mg −1 cell protein and the choline incorporated could be recovered in the soluble fraction as free choline, phosphorylcholine and CDP-choline. Choline accumulation...
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