Nanotubes modification for orthopedic implants has shown interesting biological performances (such as improving cell adhesion, cell differentiation, and enhancing osseointegration). The purpose of this study is to investigate effect of titanium dioxide (TiO 2 ) nanotube feature on performance of hydroxyapatite-coated titanium (Ti) bone implants. TiO 2 nanotubes were prepared by anodization using ammonium fluoride electrolyte (NH 4 F) with and without modifiers (PEG400 and Glycerol) at various potential forms, and times. After anodization, the nanotubes were subsequently annealed. TiO 2 nanotubes were characterized by scanning electron microscope and X-ray diffractometer. The amorphous to anatase transformation due to annealing was observed. Smooth and highly organized TiO 2 nanotubes were found when high viscous electrolyte, NH 4 F in glycerol, was used. Negative voltage (−4V) during anodization was confirmed to increase nanotube thickness. Length of the TiO 2 nanotubes was significantly increased by times. The TiO 2 nanotube was electrodeposited with hydroxyapatite (HA) and its adhesion was estimated by adhesive tape test. The result showed that nanotubes with the tube length of 560nm showed excellent adhesion. The coated HA were tested for biological test by live/dead cell straining. HA coated on TiO 2 nanotubes showed higher cells density, higher live cells, and more spreading of MC3T3-E1 cells than that growing on titanium plate surface.