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We examined neuronal differentiation of F9 teratocarcinoma cells using retinoic acid (RA) and cyclic AMP (cAMP) as inducing agents. Neuronal differentiation was monitored using (1) cDNA probes for the rat 68-kDa neurofilament gene, (2) RT-PCR for neurofilament genes and (3) antibodies against several neuronal differentiation markers. We found by Northern blotting that the uninduced F9 cells, grown...
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