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Direct exposure of human hepatoma cell line SMMC-7721 to hydrogen peroxide (H 2 O 2 ) can induce apoptosis. Apoptosis induced by H 2 O 2 was inhibited by cycloheximide, actinomycin D, 3-aminobenzamide, EGTA or Zn 2+ . H 2 O 2 can increase the level of intracellular Ca 2+ , downregulate GSH levels, slightly induce lipid peroxidation, and...
Intracellular 2,7-dichlorofluorescin (H 2 DCF) oxidation is often used to measure the production of reactive oxygen species (ROS) within cells. The rate of H 2 DCF oxidation depends on the concentration of glutathione, which is an alternative target for ROS. Our results suggest that increased rate of H 2 DCF oxidation be interpreted as an indication of general oxidative stress...
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