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A proteomic analysis combining peptide de novo sequencing and BLAST analysis was used to identify novel proteins involved in copper tolerance in the marine alga Scytosiphon gracilis (Phaeophyceae). Algal material was cultivated in seawater without copper (control) or supplemented with 100μgL −1 for 4 days, and protein extracts were separated by two-dimensional gel electrophoresis (2-DE). From...
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