The present investigation describes the Insitu removal of Cr (III) from contaminated soil by cationic biosurfactant (CBS) produced from Alcaligenes aquatilis sp. using chicken tallow as the substrate. The cationic nature of the surfactant was confirmed through amino acid composition. The SDS-PAGE revealed that the molecular weight of the biosurfactant was 100.0 KDa. The CBS was immobilized in nano porous activated carbon and the matrix was characterized using SEM, FT-IR and XRD. The CBS immobilized Nano porous activated carbon could be considered for exchange of chromium from contaminated soil and the matrix could be reused for many numbers of cycles.