The article focuses on the application of Corporate Social Responsibility (CSR) in enterprises in the Czech Republic and aims at the knowledge of the concept of Corporate Social Responsibility and engagement of companies within the particular CSR's activities. The knowledge and awareness in the field of CSR is compared to the application of the concept of CSR and the fulfilment of its three pillars – social, economic and environmental, in terms of frequency of use of particular activities and engagement in the concept of CSR as a whole. The examined group includes 702 companies from the Czech Republic; which equally represents companies from each region. The dependence between the knowledge of the concept of CSR and its application in practice is evaluated using statistical methods. The testing uses test ÷2.