The aim of this study was to investigate the possible protective effect of sodium copper chlorophyllin (CHL) against cytotoxicity and DNA damage induced by mycotoxins ochratoxin A (OTA) and fumonisin B 1 (FB 1 ). CHL (0.1–100μg/ml) alone had no impact on cell viability and genome damage in the primary human peripheral blood lymphocytes (HPBLs) and exhibited free radical scavenging activity in the DPPH assay. Both mycotoxins, OTA (4μmol/l) and FB 1 (20μg/ml), induced DNA damage in HPBLs already after 1h exposure. When the HPBLs were co-exposed to CHL (10 and 100μg/ml) and OTA (4μmol/l) or FB 1 (20μg/ml) for 1h, CHL protected against cell and DNA damage induced by both mycotoxins, implying that OTA and FB 1 cytogenotoxicity mechanisms function at least partially through oxidative stress. Therefore, CHL could be a perfect candidate for possible use as an antioxidant.