An arable soil with organic matter formed from C 3 -vegetation was amended initially with maize cellulose (C 4 -cellulose) and sugarcane sucrose (C 4 -sucrose) in a 67-day laboratory incubation experiment with microcosms at 25°C. The amount and isotopic composition ( 13 C/ 12 C) of soil organic C, CO 2 evolved, microbial biomass C, and microbial residue C were determined to prove whether the formation of microbial residues depends on the quality of the added C source adjusted with NH 4 NO 3 to the same C/N ratio of 15. In a subsequent step, C 3 -cellulose (3mgCg −1 soil) was added without N to soil to determine whether the microbial residues formed initially from C 4 -substrate are preferentially decomposed to maintain the N-demand of the soil microbial community. At the end of the experiment, 23% of the two C 4 -substrates added was left in the soil, while 3% and 4% of the added C 4 -cellulose and C 4 -sucrose, respectively, were found in the microbial biomass. The addition of the two C 4 -substrates caused a significant 100% increase in C 3 -derived CO 2 evolution during the 5–33 day incubation period. The addition of C 3 -cellulose caused a significant 50% increase in C 4 -derived CO 2 evolution during the 38–67 day incubation period. The decrease in microbial biomass C 4 -C accounted for roughly 60% of this increase. Cellulose addition promoted microorganisms strongly able to recycle N immediately from their own tissue by “cryptic growth” instead of incorporating NO 3 − from the soil solution. The differences in quality of the microbial residues produced by C 4 -cellulose and C 4 -sucrose decomposing microorganisms are also reflected by the difference in the rates of CO 2 evolution, but not in the rates of net N mineralization.