The objective of this study was to compare the effectiveness of different concentrations of ethanol treatment for activation of oocytes and their developmental potency in vitro. Ovaries were collected from a local abattoir and transported within 4h to the laboratory in warm saline (37°C) containing 100IU penicillin-G and 100μg streptomycin sulphate per ml. A total of 2680 cumulus oocyte complexes (COCs) were collected from 899 ovaries. Oocytes were matured in TCM-199 medium containing FSH (5μg/ml), LH (10μg/ml), supplemented with 20% fetal bovine serum at 38.5°C and 5% CO 2 in an incubator under humidified air for 27h. After 27h of IVM, oocytes were denuded, washed and randomly divided into five groups. Group 1 consisted of in vitro matured oocytes (n=403) as control which were washed with KSOM medium without ethanol. Group 2 consisted of in vitro matured oocytes (n=412) activated with 1% ethanol for 5min in KSOM medium. Group 3 was comprised of in vitro matured oocytes (n=362), activated with 1% ethanol for 5min in KSOM medium. Group 4 was comprised of in vitro matured oocytes (n=564) activated with 5% ethanol for 5min in KSOM medium. Group 5 consisted of in vitro matured oocytes (n=634) activated with 7% ethanol for 5min in KSOM medium. Group 6 consisted of in vitro matured oocytes (n=305) activated with 9% ethanol for 5min in KSOM medium. After 5min activation, the oocytes were washed 5–10 times in the culture medium (KSOM) and cultured in 50μl drop of KSOM. Development of activated oocytes was observed at every 24h till day 10 post insemination under inverted phase contrast microscope (200×, Nikon, Japan). The percentage of cleavage and morula production in groups 1, 2, 3, 4, 5 and 6 were 0.00% and 0.00%, 0.00% and 0.00%, 8.28% and 6.66%, 10.43% and 26.31%, 33.19% and 29.26%, 40.32% and 14.63%, respectively. These results suggested that the activation of in vitro matured oocytes by 7% ethanol for 5min in KSOM is most favorable for parthenogenetic caprine embryos production.