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The structure of [ 113 Cd 7 ]-metallothionein (MT_nc) of the Antarctic fish Notothenia coriiceps, the first three-dimensional structure of a fish metallothionein, was determined by homonuclear 1 H NMR experiments and heteronuclear [ 1 H, 113 Cd]-correlation spectroscopy. MT_nc is composed of an N-terminal β domain with 9 cysteines and 3 metal...
De novo sequence design of foldable proteins provides a way of investigating principles of protein architecture. We performed fully automated sequence design for a target structure having a three-helix bundle topology and synthesized the designed sequences. Our design principle is different from the conventional approach, in that instead of optimizing interactions within the target structure, we design...
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