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Background: Eukaryotic initiation factor 5A (elF-5A) contains an unusual amino acid, hypusine [N ε -(4-aminobutyl-2-hydroxy)lysine]. The first step in the post-translational formation of hypusine is catalysed by the enzyme deoxyhypusine synthase (DHS). The modified version of elF-5A, and DHS, are required for eukaryotic cell proliferation. Knowledge of the three-dimensional structure of this...
Background: Replication of the ColE1 plasmid of Escherichia coli is regulated by the interaction of sense and antisense plasmid-encoded transcripts. The antisense RNA I negatively regulates the replication of the plasmid by duplex formation with complementary RNA II. The interaction is initiated by the formation of a double helix between seven-nucleotide loops from each RNA and is stabilized by binding...
Background: The four members of the INK4 gene family (p16 INK4a , p15 INK4b , p18 INK4c and p19 INK4d ) inhibit the closely related cyclin-dependent kinases CDK4 and CDK6 as part of the regulation of the G 1 ->S transition in the cell-division cycle. Loss of INK4 gene product...
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