Activation of T lymphocytes requires protein kinase C theta (PKC-θ) and an appropriately elevated free intracellular Ca 2+ concentration ([Ca 2+ ] i ). Here, we show that phorbol 12 myristate 13-acetate (PMA) inhibited Ca 2+ influx in wild-type but not PKC-θ −/− T cells, suggesting that PKC-θ plays a role in PMA-mediated inhibition of Ca 2+ influx. In contrast, T cell receptor (TCR) crosslinking in the same PKC-θ −/− T cells did result in significantly decreased [Ca 2+ ] i compared to wild-type T cells, suggesting a positive role for PKC-θ in TCR-mediated Ca 2+ mobilization. In PKC-θ −/− mice, peripheral mature T cells, but not developing thymocytes, displayed significantly decreased TCR-induced Ca 2+ influx and nuclear factor of activated T cells (NFAT) translocation upon sub-optimal TCR crosslinking. The decreased intracellular free Ca 2+ was due to changes in Ca 2+ influx but not efflux, as observed in extracellular and intracellular Ca 2+ mobilization studies. However, these differences in Ca 2+ influx and nuclear factor of activated T cells (NFAT) translocation disappeared with increasing intensity of TCR crosslinking. The enhancing effect of PKC-θ on Ca 2+ influx is not only dependent on the strength of TCR crosslinking but also on the developmental stage of T cells. The underlying mechanism involved phospholipase Cγ1 activation and inositol triphosphate production. Furthermore, knockdown of endogenous PKC-θ expression in Jurkat cells resulted in significant inhibition of TCR-induced activation of NFAT, as evidenced from NFAT reporter studies. Forced expression of a constitutively active form of calcineurin in PKC-θ −/− Jurkat cells could readily overcome the above inhibition. Thus, PKC-θ can both positively and negatively regulate the Ca 2+ influx that is critical for NFAT activity.