In order to assess the patterns of linkage disequilibrium (LD) about 67 SNP loci on X chromosome, genomic DNA samples extracted from blood fluids from 213 unrelated Chinese Han female individuals were analyzed through multiplex amplification followed by MALDI-TOF MS. LD was analyzed by SNPAnalyzer 2.0. Slight LD were found at two pairs and tight LD were observed at six pairs. From the 67 X-SNP loci, 56 informative X-SNPs showing independent inheritance could be chosen out. Both of the combination discrimination power and the cumulative exclusion probability of the 56 markers were above 0.999999. It may be concluded that the panel of 56 X-SNPs loci is valuable in forensic identification.