It takes a long time from start to reach a comfortable indoor temperature when spit air conditioner is heating, the vertical stratification and thermal comfort is bad after the thermal stability. In the light of the above phenomenon. This paper comparative analyzed the indoor temperature distribution and human comfort conditions by experiment in three supply air condition of normal operating, with single supply air temperature and the supply air combination project. The results indicate that the higher the supply air temperature, the faster the indoor average temperature raise, but the indoor thermal stratification was serious, lower air temperatures within a certain range will help improve the temperature of low plane and improve indoor thermal comfort, but the indoor average temperature rises slowly,. However, the combination scheme not only improves the average indoor temperature as soon as possible, but also effectively alleviate the indoor thermal stratification, people feel more comfortable. The effect of different combination schemes are different, among them, the air supply scheme of supplying the high temperature first and then supplying the low temperature is best in effect of improve indoor thermal stratification and distribution of indoor air.