The interaction between tumor cells and laminin mediated by laminin-binding integrins is critical for tumor invasion and metastasis. The aim of this study was to clarify the altered expression of lamininbinding integrins with the change of laniinin deposition in hepatocellular carcinoma (HCC) in comparison with cirrhotic or normal liver by immunohistochemistry. In HCC, hepatoma cells and sinusoidal endothelial cells expressed integrins α1β1, α2β1, α3β1, and α6β1. Integrins α1β1 and α6β1 were detected in a continuous pattern along the sinusoids in accordance with laminin assembly. Integrins α2β1 and α3β1 were detected in a discontinuous pattern at these sites. Integrin α6β4 was not detected. In cirrhotic liver, although integrins α1β1 and α6β1 as well as laminin were detected in a continuous pattern along the sinusoids, integrins α2β1, α3β1, and α6β4 were not detected. In normal liver, although integrin α1β1 was detected in a continuous pattern along the sinusoids, neither integrins α2β1, α3β1, α6β1, α6β4, nor laminin were detected. We have clarified that, of laminin-binding integrins, the localization of integrin α6β1 shows the best correspondence with the localization of laminin. These results suggest that of laminin-binding integrins, integrin α6β1 is very important for cell-laminin interactions in HCC.