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The conformation in solution of the biantennary complex type oligosaccharide unit derived from human serotransferrin has been investigated using 1 H- 1 H Nuclear Overhauser Effect (NOE) measurements at 300 MHz. From quantitation of the NOE, the α(1-3) antenna is shown to exist in a preferred solution conformation with respect to the mannosyl-chitobiose core. The flexibility of the...
The effects of varied durations of food deprivation on the rates and kinetics of glucose phosphorylation by isolated rat hepatocytes have been examined. Glucokinase activity was measured concurrently in extracts from these cells prepared from livers of rats which had fasted for 0, 24, 48 and 72 h. Significant levels of hepatocyte glucose phosphorylation were noted even when glucokinase levels were...
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