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Bovine heart mitochondrial transhydrogenase, a redox-linked proton pump, can be functionally and asymmetrically inserted into liposomes by a cholate-dialysis procedure such that the active site faces the external medium. N-(4-Azido-2-nitrophenyl)-2-aminoethylsulfonate (NAP-taurine), a membrane-impermeant photoprobe, when encapsulated in the vesicles, covalently modified the enzyme and inhibited transhydrogenation...
Electrofusion of mesophyll protoplasts of Avena sativa was induced by using electromagnetic coupling of energy including that from a spark discharge. The results suggest that electrofusion may have played an important role in the evolutionary process and that new species arose in this way by electromagnetic waves emitted by lightning in thunderstorms. If cell fusion had occurred in prehistory by the...
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