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The effect of microtubule associated proteins on microtubule shape has been investigated in reconstitution experiments using purified tubulin and purified MAP1A, MAP1B, and MAP2. Microtubules assembled in the presence of these MAPs were fixed with 0.1% glutaraldehyde and, after negative staining, were examined by electron microscopy. The results show that MAP1A microtubules were generally short...
The effect of estramustine and estramustine phosphate (EP) on the interaction of microtubule associated protein 2 (MAP2) with actin has been examined. We show that (a) neither estramustine nor EP influences actin polymerisation (b) EP, but not estramustine, reduces the amount of MAP2 which co-sediments with F-actin in a dose-dependent manner and (c) EP decreases the MAP2-induced crosslinking of F-actin...
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