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A large (65%) fraction of in vitro cultured rat chromaffin cells exhibit spontaneous [Ca 2+ ] i oscillations, and the rest can be recruited to oscillate by appropriate stimulations. Based on fura-2 single cell [ca 2+ ] i measurements, evidence is provided that these oscillation originate, via the activation of Ca 2+ -induced Ca 2+ ...
The possibility that the ml muscarinic receptor subtype can induce release of intracellular granules and transmitters was studied by transfecting a cultured mast cell line, RBL-2H3 cells, with the ml receptor gene. Comparisons were made between carbachol- and antigen-induced activation of various secretory responses. Like antigen, carbachol stimulated inositol phospholipid hydrolysis and release of...
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