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Polyclonal antibodies have been raised to synthetic amino acid sequences of the bovine GABA A receptor α 1 and α 3 subunits. Anti-α 1 subunit antibodies recognise a polypeptide of 53 kDa whereas anti-α 1 subunit antibodies recognise a polypeptide of 59-60 kDa, in Western blots of GABA A receptor purified from adult bovine cerebral cortex, cerebellum...
The isoquinoline carboxamide derivative [ 3 H]PK11195, a ligand for the peripheral-type benzodiazepine (BZD) receptor, binds to Chinese hamster ovary (CHO) cell mitochondria in a specific and saturable manner. Scatchard analysis showed the presence of a single-binding site with an apparent dissociation constant (K d ) of 12.0 +/- 1.0 nM and a maximal binding capacity of 23.0 +/- 2...
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