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Cumene hydroperoxide (230 μM)-induced fall of the membrane potential takes place only in Ca 2+ -loaded mitochondria. Inhibitor of phospholipase A 2 , P-bromphenacyl bromide prevents uncoupling of mitochondria, having no effect on the accumulation of lipid peroxidation products.
In this study we demonstrate that: (1) although the major phospholipase A 2 present in sheep platelets is activated by calcium ions, it can effectively catalyze hydrolysis of the sn-2 ester linkage in phospholipids in the absence of calcium: (2) expression of calcium-independent phospholipase A 2 activity can be induced by NACl utilizing purified (but not crude) cytosolic enzyme; and...
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