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rtPCR and Western blotting were used to determine which members of the PKC family are present in both immature and mature mouse oocytes. Using isoform-specific PCR primers and antibodies PKC-δ and -λ were detected while such techniques failed to observe the conventional isoforms of PKC-α, -β, -γ. This isoform profile was confirmed using an alternative PCR strategy, which allowed discrimination of...
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