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The Saccharomyces cerevisiae Ras2p has been suggested to be a target in the feedback regulation of Ras-cAMP pathway. This work proves that the Ras2p localization is regulated by PKA activity, and that PKA down-regulates Ras2p activity and the protein association between Cdc25p and Ras2-GTP, which is due to a reduced Ras2-GEF Cdc25p activity. These results suggest that Ras2p localization and Ras2-GEF...
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