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Stably transfected HEK-293 cells express on their surface the murine type II IL-1 receptor (mIL-1RII) as demonstrated by FACS analysis using the mAb 4E2, however binding of [ 125 I]-hrIL-1β to these cells is nearly absent. Saturable high affinity binding of [ 125 I]-hrIL-1β is observed when the murine IL-1 receptor accessory protein (mIL-1RAcP) is coexpressed with...
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