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Brain D 2 dopamine receptors have been solubilised using lysophosphatidylcholine. The inclusion of proteinase inhibitors during solubilisation enables a preparation to be obtained containing a high proportion of solubilised D 2 receptors with pharmacological properties similar to those of membrane bound D 2 receptors.
Solubilisation, delipidation and partial purification of the membrane-bound enzyme. D-amino acid dehydrogenase of Escherichia coli K 12 produced significant changes in several of its properties. Solubilised enzyme showed a broader substrate specificity, increased affinity for at least three substrates, and a lower pH optimum with D-alanine as substrate. Solubilised enzyme was more heat-labile than...
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