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In our previous work (Mansilla et al. (1997) Protein Eng. 10, 927-934) we showed that Arg 7 of Escherichia coli elongation factor Tu (EF1A) plays an essential role in aminoacyl-tRNA (aa-tRNA) binding. Substitution of Arg 7 by Ala or Glu lost this activity. We proposed that Arg 7 forms a salt bridge with the charged conserved amino acid Glu 272 (Asp 2 ...
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