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The tubulins of the plant Phaseolus vulgaris have been identified in cell extracts by a combination of western blotting of 1D and 2D gels and detection by monoclonal antibodies specific for α- and β-tubulin. Plant α-tubulin differs from mammalian tubulin in that it migrates ahead of the β-subunit on Laemmli SDS gels. The Phaseolus root tip extracts contain 4 α- and 4 β-tubulin isotypes which are electrophoretically...
We have used a panel of monoclonal antibodies in a study of the expression of multiple tubulins in Physarum polycephalum. Three anti-β-tubulin monoclonal antibodies, DM 1B, DM3B3 and KMX-1 all reacted with the β 1 -tubulin isotypes expressed in both myxamoebae and plasmodia. However, these antibodies showed a spectrum of reduced reactivity with the plasmodial β 2 -tubulin isotype -...
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