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We have used 19 F NMR and CD spectra to study interactions of TFP with CaM under various conditions where no aggregation of TFP occurs. It was found that TFP is bound to CaM in the absence of Ca 2+ with K a >4 x 10 4 M in terms of 19 F NMR of TFP and that KCl markedly influences the interaction of TFP with CaM. It was also found that TFP causes...
We have used 19 F NMR to study interactions of trifluoperazine (TFP), a potent calmodulin (CaM) antagonist, with Tetrahymena calmodulin (Tet. CaM). Changes in chemical shift and bandwidth of TFP caused by adding Tet. CaM in the presence of excess Ca 2+ were much smaller than those by adding porcine CaM. The spectral features of the TFP-Tet. CaM solution in the presence of...
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