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The effect on the structure of profilin of phosphatidylinositol 4,5-bisphosphate (PIP 2 ) binding was probed by fluorescence and circular dichroism (CD) spectroscopy, Fluorescence of Trp 3 and Trp 31 of profilin at 292 nm showed a linear decrease in solution emission at 340 nm as PIP 2 /profilin was increased from 0 to 80:1, apparently due to a static quenching...
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